Mutations within the Open Reading Frame (ORF) including Ochre stop codon of the Surface Glycoprotein gene of SARS-CoV-2 virus erase potential seed location motifs of human non-coding microRNAs

MicroRNA are short and non-coding RNA, 18-25 nucleotides in length. They produced at the early stage of viral infection. The roles played by cellular miRNAs miRNA-mediated gene-silencing COVID-19 epidemic period is critical order to develop novel therapeutics. We analyzed SARS-CoV-2 Surface Glycoprotein (S) nucleotide sequence originating from India as well Iran, Australia, Germany, Italy, Russia, China, Japan Turkey identified mutation potential seed location several human miRNA. Seventy single polymorphisms (SNP) were detected S gene out which, 36, 32 2 cases transitions, transversions deletions respectively. Eleven miRNA targets on reference with a score >80 miRDB database. Mutation A845S erased common binding site 7 (miR-195-5p, miR-16-5p, miR-15b-5p, miR-15a-5p, miR-497-5p, miR-424-5p miR-6838-5p). A synonymous altered wild type Ochre stop codon within (Italy) Opal thereby changing miR-511-3p. Similar (synonymous) mutations amino acid position 659 1116 where acids serine threonine retained, abolishing for miR-219a-1-3p miR-20b-3p significance this finding strategy use synthetic combinations therapeutic tool discussed.